Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).
Identifieur interne : 000082 ( Main/Exploration ); précédent : 000081; suivant : 000083Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).
Auteurs : Mohamad Fadhli Bin Mad' Atari [États-Unis, Malaisie] ; Kevin M. Folta [États-Unis]Source :
- BMC research notes [ 1756-0500 ] ; 2019.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Arabidopsis (génétique), Feuilles de plante (génétique), Fragaria (génétique), Génie génétique (instrumentation), Génie génétique (méthodes), Reproductibilité des résultats (MeSH), Tabac (génétique), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Agrobacterium tumefaciens, Arabidopsis, Feuilles de plante, Fragaria, Tabac.
- méthodes : Génie génétique.
- instrumentation : Génie génétique, Reproductibilité des résultats, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Arabidopsis (genetics), Fragaria (genetics), Genetic Engineering (instrumentation), Genetic Engineering (methods), Plant Leaves (genetics), Plants, Genetically Modified (MeSH), Reproducibility of Results (MeSH), Tobacco (genetics), Transformation, Genetic (MeSH).
- MESH :
- genetics : Agrobacterium tumefaciens, Arabidopsis, Fragaria, Plant Leaves, Tobacco.
- instrumentation : Genetic Engineering.
- methods : Genetic Engineering.
- Plants, Genetically Modified, Reproducibility of Results, Transformation, Genetic.
Abstract
OBJECTIVE
The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.
RESULTS
We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.
DOI: 10.1186/s13104-019-4117-3
PubMed: 30876440
PubMed Central: PMC6419847
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Folta</name><affiliation wicri:level="1"><nlm:affiliation>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611, USA. kfolta@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30876440</idno><idno type="pmid">30876440</idno><idno type="doi">10.1186/s13104-019-4117-3</idno><idno type="pmc">PMC6419847</idno><idno type="wicri:Area/Main/Corpus">000117</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000117</idno><idno type="wicri:Area/Main/Curation">000117</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000117</idno><idno type="wicri:Area/Main/Exploration">000117</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).</title><author><name sortKey="Mad Atari, Mohamad Fadhli Bin" sort="Mad Atari, Mohamad Fadhli Bin" uniqKey="Mad Atari M" first="Mohamad Fadhli Bin" last="Mad' Atari">Mohamad Fadhli Bin Mad' Atari</name><affiliation wicri:level="1"><nlm:affiliation>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.</nlm:affiliation><country xml:lang="fr">Malaisie</country><wicri:regionArea>School of Biological Sciences, Universiti Sains Malaysia, 11800, Penang</wicri:regionArea><wicri:noRegion>Penang</wicri:noRegion></affiliation></author><author><name sortKey="Folta, Kevin M" sort="Folta, Kevin M" uniqKey="Folta K" first="Kevin M" last="Folta">Kevin M. Folta</name><affiliation wicri:level="1"><nlm:affiliation>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611, USA. kfolta@ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Horticultural Sciences Department, University of Florida, 1301 Fifield Hall, PO Box 110690, Gainesville, FL, 32611</wicri:regionArea><wicri:noRegion>32611</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC research notes</title><idno type="eISSN">1756-0500</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Arabidopsis (genetics)</term><term>Fragaria (genetics)</term><term>Genetic Engineering (instrumentation)</term><term>Genetic Engineering (methods)</term><term>Plant Leaves (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Reproducibility of Results (MeSH)</term><term>Tobacco (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Arabidopsis (génétique)</term><term>Feuilles de plante (génétique)</term><term>Fragaria (génétique)</term><term>Génie génétique (instrumentation)</term><term>Génie génétique (méthodes)</term><term>Reproductibilité des résultats (MeSH)</term><term>Tabac (génétique)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Fragaria</term><term>Plant Leaves</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Arabidopsis</term><term>Feuilles de plante</term><term>Fragaria</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie génétique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Plants, Genetically Modified</term><term>Reproducibility of Results</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Génie génétique</term><term>Reproductibilité des résultats</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>OBJECTIVE</b></p><p>The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. The development of the Standardized Pressure Agrobacterium Infiltration Device (SPAID) provides a means to deliver consistent amounts of bacterial suspensions into plant tissues with the goal of increasing reproducibility between replicates and laboratories.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30876440</PMID><DateCompleted><Year>2019</Year><Month>07</Month><Day>08</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1756-0500</ISSN><JournalIssue CitedMedium="Internet"><Volume>12</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>Mar</Month><Day>15</Day></PubDate></JournalIssue><Title>BMC research notes</Title><ISOAbbreviation>BMC Res Notes</ISOAbbreviation></Journal><ArticleTitle>Transformation improvement with the Standardized Pressure Agrobacterium Infiltration Device (SPAID).</ArticleTitle><Pagination><MedlinePgn>144</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13104-019-4117-3</ELocationID><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">The treatment of plant tissue with Agrobacterium tumefaciens is often a critical first step to both stable and transient plant transformation. In both applications bacterial suspensions are oftentimes physically introduced into plant tissues using hand-driven pressure from a needleless syringe. While effective, this approach has several drawbacks that limit reproducibility. Pressure must be provided with the syringe perfectly perpendicular to the tissue surface. The researcher must also attempt to provide even and consistent pressure, both within and between experimental replicates. These factors mean that the procedures do not always translate well between research groups or biological replicates.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">We have devised a method to introduce Agrobacterium suspensions into plant leaves with greater reproducibility. Using a decommissioned dissecting microscope as an armature, a syringe body with the bacterial suspension is mounted to the nosepiece. Gentle, even pressure is applied by rotating the focus knob. The treatment force is measured using a basic kitchen scale. 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name="États-Unis"><noRegion><name sortKey="Mad Atari, Mohamad Fadhli Bin" sort="Mad Atari, Mohamad Fadhli Bin" uniqKey="Mad Atari M" first="Mohamad Fadhli Bin" last="Mad' Atari">Mohamad Fadhli Bin Mad' Atari</name></noRegion><name sortKey="Folta, Kevin M" sort="Folta, Kevin M" uniqKey="Folta K" first="Kevin M" last="Folta">Kevin M. Folta</name></country><country name="Malaisie"><noRegion><name sortKey="Mad Atari, Mohamad Fadhli Bin" sort="Mad Atari, Mohamad Fadhli Bin" uniqKey="Mad Atari M" first="Mohamad Fadhli Bin" last="Mad' Atari">Mohamad Fadhli Bin Mad' Atari</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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